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RESPIRATORY

2SmartFinder® technology

Single tube Multiplex Amplification in Real Time

RespiFinder® 2Smart

PF2600-2S

RespiFinder® 2Smart

Multiplex real-time PCR for syndromic approach of respiratory tract infection diagnostics. Simultaneous detection of 20 viral and 4 bacterial pathogens.
 

Targets:

​

Viruses

  • Influenza A

  • Influenza B

  • Influenza A(H1N1)pdm09

  • RSV-A

  • RSV-B

  • Human Metapneumovirus

  • Rhinovirus/Enterovirus

  • Adenovirus

  • Parainfluenza-1

  • Parainfluenza-2

  • Parainfluenza-3

  • Parainfluenza-4

  • Bocavirus

  • Coronavirus NL63/HKU1

  • Coronavirus OC43

  • Coronavirus 229E

  • SARS-CoV-2

  • MERS CoV
     

Bacteria

  • Mycoplasma pneumoniae

  • Chlamydophila pneumoniae

  • Legionella pneumophila

  • Bordetella pertussis
     

Features and benefits:

  • 20 viral and 4 bacterial Respiratory tract infection

       causing pathogens in one assay

  • Diagnosis in 2.5 hours after nucleic acid extraction

  • Detection based on melting curve analysis

  • As sensitive as monoplex real-time PCR

  • Internal Control included

  • Amplification Controls included

  • Positive Control included

  • Interpretation software available

Validated diagnostic specimens:

  • Nasopharyngeal swab
     

Validated detection systems:

  • LightCycler® 480 (Roche)

  • Rotor-Gene® Q(QIAGEN)
     

Validated extraction methodes:

  • NucliSENS® easyMAG® (bioMérieux)

  • QIAamp® MinElute Virus Spin Kit (QIAGEN)

  • QIAsymphony (QIAGEN)

RealAccurate® Quadruplex Influenza PCR Kit

RealAccurate® Quadruplex Influenza PCR Kit

PF0970-R

Real-time PCR kit to detect and differentiate Influenza A, Influenza B and Influenza A (H1N1)pdm09

​

Targets:

  • Influenza A virus

  • Influenza B virus

  • Influenza A(H1N1)pdm09

​

Features and benefits:

  • Can be combined with other Respiratory RealAccurate® kits

  • Internal Control included

  • Positive Control included

  • Sample to result within 2.5 hours

​

Validated diagnostic specimens:

  • Nasopharyngeal swabs

​

Validated detection systems:

  • LightCycler® 480 (Roche)

  • Rotor-Gene® Q (QIAGEN)

  • CFX96™ (Bio-Rad)

  • Mic qPCR Cycler (Bio Molecular Systems)

  • QuantStudio™ 5(ThermoFisher)

​

Validated extraction methodes:

  • NucliSENS® easyMAG® (bioMérieux)

  • InviGenius® PLUS (Invitek Molecular)

RealAccurate® Quadruplex Corona PCR Kit

RealAccurate® Quadruplex Corona PCR Kit

PF0971-R

Real-time PCR kit to detect and distinguish four Coronavirus subtypes

​

Targets:

  • Coronavirus 229E

  • Coronavirus OC43

  • Coronavirus NL63/HKU1

​

Features and benefits:

  • Can be combined with other Respiratory RealAccurate® kits

  • Internal Control included

  • Positive Control included

  • Sample to result within 2.5 hours

​

Validated diagnostic specimens:

  • Nasopharyngeal swabs

​

Validated detection systems:

  • LightCycler® 480 (Roche)

  • Rotor-Gene® Q (QIAGEN)

  • CFX96™ (Bio-Rad)

  • Mic qPCR Cycler (Bio Molecular Systems)

  • QuantStudio™ 5(ThermoFisher)

​

Validated extraction methodes:

  • NucliSENS® easyMAG® (bioMérieux)

  • InviGenius® PLUS (Invitek Molecular)

RealAccurate® Quadruplex SARS-CoV-2 PCR Kit

RealAccurate® Quadruplex SARS-CoV-2 PCR Kit

PF0971C-R

Real-time PCR kit to detect and confirm SARS-CoV-2 coronavirus infection.

​

Targets:

  • SARS-CoV-2 N gene

  • SARS-CoV-2 RdRp gene

‍

Features and benefits:

  • Can be combined with other Respiratory RealAccurate® kits

  • Internal Control included

  • Positive Control included

  • Sample to result within 2.5 hours

‍

Validated diagnostic specimens:

  • Nasopharyngeal swabs

‍

Validated detection systems:

  • LightCycler® 480 (Roche)

  • Rotor-Gene® Q (QIAGEN)

  • CFX96™ (Bio-Rad)

  • Mic qPCR Cycler (Bio Molecular Systems)

  • QuantStudio™ 5(ThermoFisher)

  • MyGoPro ESR (IT-IS Life Science)

‍

Validated extraction methodes:

  • NucliSENS® easyMAG® (bioMérieux)

  • Heat-treatment samples in UTM and eSwab medium

RealAccurate® Quadruplex Parainfluenza PCR Kit

RealAccurate® Quadruplex Parainfluenza PCR Kit

PF0972-R

Real-time PCR kit to detect and distinguish four Parainfluenza subtypes.

​

Targets:

  • Parainfluenza 1

  • Parainfluenza 2/4

  • Parainfluenza 3

‍

Features and benefits:

  • Can be combined with other Respiratory RealAccurate® kits

  • Internal Control included

  • Positive Control included

  • Sample to result within 2.5 hours

‍

Validated diagnostic specimens:

  • Nasopharyngeal swabs

‍

Validated detection systems:

  • LightCycler® 480 (Roche)

  • Rotor-Gene® Q (QIAGEN)

  • CFX96™ (Bio-Rad)

  • Mic qPCR Cycler (Bio Molecular Systems)

  • QuantStudio™ 5(ThermoFisher)

‍

Validated extraction methodes:

  • NucliSENS® easyMAG® (bioMérieux)

  • InviGenius® PLUS (Invitek Molecular)

RealAccurate® Quadruplex RSV/hMPV PCR Kit

RealAccurate® Quadruplex RSV/hMPV PCR Kit

PF0973-R

Real-time PCR kit to detect Respiratory Syncitial Virus subtypes A and B and Human Metapneumovirus.

​

Targets:

  • Respiratory Syncytial Virus A

  • Respiratory Syncytial Virus B

  • Human Metapneumovirus A/B

‍

Features and benefits:

  • Can be combined with other Respiratory RealAccurate® kits

  • Internal Control included

  • Positive Control included

  • Sample to result within 2.5 hours.

‍

Validated diagnostic specimens:

  • Nasopharyngeal swabs

‍

Validated detection systems:

  • LightCycler® 480 (Roche)

  • Rotor-Gene® Q (QIAGEN)

  • CFX96™ (Bio-Rad)

  • Mic qPCR Cycler (Bio Molecular Systems)

  • QuantStudio™ 5(ThermoFisher)

‍

Validated extraction methodes:

  • NucliSENS® easyMAG® (bioMérieux)

  • InviGenius® PLUS (Invitek Molecular)

RealAccurate® Quadruplex Adeno/Boca/Rhino/Entero PCR Kit

RealAccurate® Quadruplex Adeno/Boca/Rhino/Entero PCR Kit

PF0974-R

Real-time PCR kit to detect Adenovirus, Bocavirus and Rhino/Enterovirus

​

Targets:

  • Adenovirus

  • Bocavirus

  • Rhinovirus/Enterovirus

‍

Features and benefits:

  • Can be combined with other Respiratory RealAccurate® kits

  • Internal Control included

  • Positive Control included

  • Sample to result within 2.5 hours.

‍

Validated diagnostic specimens:

  • Nasopharyngeal swabs

‍

Validated detection systems:

  • LightCycler® 480 (Roche)

  • Rotor-Gene® Q (QIAGEN)

  • CFX96™ (Bio-Rad)

  • Mic qPCR Cycler (Bio Molecular Systems)

  • QuantStudio™ 5(ThermoFisher)

‍

Validated extraction methodes:

  • NucliSENS®easyMAG® (bioMérieux)

  • InviGenius® PLUS (Invitek Molecular)

RealAccurate® Quadruplex Mycobacteria PCR Kit

RealAccurate® Quadruplex Mycobacteria PCR Kit

PF0920-R

Differentiation between Mycobacterium tuberculosis complex and clinical significant nontuberculous mycobacteria (NTM)

​

Targets:

  • Mycobacterium tuberculosis complex

  • Nontuberculous mycobacteria

‍

Features and benefits:

  • Simultaneous detection of Mycobacterium tuberculosis complex and nontuberculous mycobacteria in one assay

  • Much faster than conventional methodology. Sample-to-result within 2 hours, as opposed to culturing (2-8 weeks)

  • Internal Control included

  • Positive Control included

  • Sample to result within 2.5 hours

‍

Validated diagnostic specimens:

  • Sputum

  • Bronchoalveolar lavage (BAL)

  • Deconaminated sediment of BAL or sputum

  • Mycobacteria Growth Indicator Tube (MGIT™) samples

‍

Validated detection systems:

  • LightCycler® 480 (Roche)

  • Rotor-Gene® Q (QIAGEN)

  • CFX96™ (Bio-Rad)

  • Mic qPCR Cycler (Bio Molecular Systems)

​

Validated extraction methodes:

  • RTP® Pathogen kit (Invitek molecular)

  • NucliSENS® easyMAG® (bioMérieux)

  • QIAamp® MinElute Virus Spin Kit (QIAGEN)

RealAccurate® Quadruplex Bordetella PCR Kit

RealAccurate® Quadruplex Bordetella PCR Kit

PF0920-R

Real-time PCR kit to detect and distinguish three Bordetella subtypes

​

Targets:

  • Bordetella pertussis

  • Bordetella parapertussis

  • Bordetella holmesii

‍

Features and benefits:

  • Discrimination between Bordetella pertussis, Bordetella parapertussis and Bordetella holmesii in one assay

  • Internal Control included

  • Positive Control included

  • More sensitive and faster than conventional culturing methodology

  • Sample to result within 2.5 hours.

‍

Validated diagnostic specimens:

  • Nasopharyngeal swabs

 

Validated detection systems:

  • LightCycler® 480 (Roche)

  • Rotor-Gene® Q (QIAGEN)

  • CFX96™ (Bio-Rad)

  • Mic qPCRCycler (Bio Molecular Systems)

​

Validated extraction methodes:

  • NucliSENS® easyMAG® (bioMérieux)

  • MagNA Pure 96 (Roche)

  • QIAamp® cador® Pathogen Mini Kit (QIAGEN)

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