MENINGITIS

Multiplex real-time PCR for syndromic approach of central nervous system infection diagnostics. Simultaneous detection of 12 viral, 8 bacterial and 2 fungal pathogens.

​

Targets:

​

Bacteria

• Listeria monocytogenes

• Staphylococcus aureus

• Haemophilus influenzae

• Streptococcus pneumoniae

• Streptococcus agalactiae

• Neisseria meningitidis

• Borrelia burgdorferi sensu lato/ Borrelia miyamotoi

• Escherichia coli K1

​

Fungi

• Cryptococcus gattii sensu lato

• Cryptococcus neoformans sensu lato

​

Viruses

• Herpes Simplex virus type 1

• Herpes Simplex virus type 2

• Varicella-zoster virus (VZV)

• Epstein-Barr Virus (EBV)

• Cytomegalovirus (CMV)

• Human herpesvirus 6

• Human herpesvirus 7

• Human herpesvirus 8

• Human enterovirus

• Parechovirus

• Mumps virus

• Measles virus

​​

Features and benefits

• 12 viral,8 bacterial and 2 fungal pathogens causing Central Nervous System (CNS) infection in one assay

• Diagnosis in 2.5 hours after nucleic acid extraction

• Detection based on melting curve analysis

• As sensitive as monoplex real-time PCR

• Internal Control included

• Amplification Controls included

• Positive Control included

• Interpretation software available

​

Validated diagnostic specimens

• Cerebrospinal fluid (CSF)

​

Validated detection systems

• LightCycler® 480 (Roche)

• Rotor-Gene® Q (QIAGEN)

​

Validated extraction methodes

• NucliSENS® easyMAG® (bioMérieux)

• QIAamp® cador® Pathogen Mini Kit (QIAGEN)

​

​