GASTROENTERITIS

Multiplex real-time PCR for syndromic approach of gastrointestinal tract infection diagnostics. Simultaneous detection of 9 bacterial, 4 parasitic and 5 viral pathogens.

​

Targets:

​

Bacteria

• Campylobacter spp.

• Salmonella spp.

• Yersinia enterocolitica

• Clostridium difficile toxin A

• Clostridium difficile toxin B

• Shiga toxin producing E. coli (STEC), stx1/stx2

• Enteropathogenic E. coli (EPEC), eae

• Enterotoxigenic E. coli (ETEC), elt/est

• Shigella/enteroinvasive E. coli (EIEC), ipaH

​

Parasites

• Entamoeba histolytica

• Giardia lamblia

• Cryptosporidium spp.

• Dientamoeba fragilis

‍

Viruses

• Norovirus (GI/GII/GIV)

• Rotavirus (A)

• Adenovirus (40/41)

• Astrovirus

• Sapovirus (GI/GII/GIV/GV)

​

Features and benefits

• 9 bacterial, 5 viral and 4 parasitic pathogens causing gastrointestinalinfection in one assay

• Diagnosis in 2.5 hours after nucleic acid extraction

• Detection based on melting curve analysis

• As sensitive as monoplex real-time PCR

• Internal Control included

• Amplification Controls included

• Positive Control included

• Interpretation software available

​

Validated diagnostic specimens

• Stool samples

​

Validated detection systems

• LightCycler® 480 (Roche)

• Rotor-Gene® Q (QIAGEN)

​

Validated extraction methodes

• NucliSENS® easyMAG® (bioMérieux)

​

​