BIODYNAMI
NGS Library Prep
NGS library prep kits
- DNA Library Prep – BioDynami NGS DNA Library Prep Kit has a simple workflows and a fast 1.5 hours protocol time, and 10 minutes of hands-on time.
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DNA Library Prep Customization – BioDynami NGS DNA Library Prep Customization Kit made library prep possible with customized adaptors and PCR primers.
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Low Input DNA Library Prep – BioDynami NGS Low Input DNA Library Prep Kit is specially developed for low input DNA from 1 ng. The fast 1.5 hour protocol make it easy for NGS library prep.
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FFPE DNA Library Prep – BioDynami NGS FFPE DNA Library Prep Kit provide a quick and easy approach of NGS library prep for FFPE DNA samples.
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Cell-free DNA Library Prep – Low amount of cell-free DNA is perfect for BioDynami’s kit. The input DNA amount starts from 1 ng.
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ChIP-Seq Library Prep – The low input amounts of ChIP-Seq library prep are compatible with BioDynami’s fast and simple workflow. Input ChIP DNA amount is from 5 ng to 400 ng.
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Single Stranded DNA Library Prep – The BioDynami Single Stranded DNA Library Prep Kit was developed for construction of high quality libraries using single stranded DNA (50 ng – 500 ng) as input.
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Ancient DNA Library Prep – The BioDynami Ancient DNA Library Prep Kit was was developed for construction of high quality libraries using ancient DNA as input. The kit is based on single stranded DNA library preparation, which is more effective for ancient DNA samples.
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Bisulfite Sequencing Library Prep – The BioDynami Bisulfite Sequencing Library Prep Kit was developed for construction of NGS libraries using bisulfite treated DNA as input. Bisulfite Sequencing Library Prep Kit overcomes the significant library loss due to the bisulfite conversion.
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Methylation Specific Bisulfite-Seq Library Prep – The BioDynami Methylation Specific Bisulfite-Seq Library Prep Kit was developed for construction of NGS libraries for methylated CpG sites using bisulfite treated DNA as input. The kit enriches methylated CpG regions, thus significantly reduce the sequencing cost. The kit estimates the whole genome methylation patterns at the single base level.
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PCR-Free NGS DNA Library Prep – The BioDynami PCR-free NGS DNA Library Prep Kit was developed for construction of high quality NGS libraries without PCR. BioDynami kit can enrich fully ligated libraries and increase the sequencing yield.
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NGS DNA Fragmentation & Library Prep – The BioDynami NGS DNA Fragmentation & Library Prep Kit was developed for construction of NGS libraries without DNA shearing equipment. The kit uses intact genomic DNA as input DNA without an additional DNA fragmentation step.
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RNA Seq Library Prep – RNA Seq Library Prep Kit was developed for construction of high quality libraries for next generation sequencing . The kit needs purified RNA (example: rRNA depleted RNA or polyA mRNA) as input for library construction.
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NGS Library Circularization Kit (MGI platform) – NGS Library Circularization Kit (MGI Platform)was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).
DNA Purification (Magnetic Beads)
Our Magnetic Beads combines BioDynami’s proprietary chemistries with reversible DNA-binding properties of SPRI Magnetic beads. The beads are developed for effective nucleic acid purification by removing impurities.
- Magnetic Beads (DNA & RNA Purification) – BioDynami standard magnetic beads for DNA and RNA purification.
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Magnetic Beads (microRNA & Oligo Purification) – BioDynami magnetic beads for purification of microDNA, short DNA fragments, short ssDNA fragments, short RNA fragments, short DNA/RNA hybrid fragments, oligos, and chimeric oligos >20 nt.
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Magnetic Beads (Short Oligo Purification)– BioDynami magnetic beads for purification of short oligos as short as 6 nt.
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Magnetic Beads (PCR Purification) – BioDynami magnetic beads for purification of PCR fragments >80 bp and removal of primers <30 nt.
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Magnetic Beads (tRNA Purification) – BioDynami magnetic beads for purification of tRNA, short DNA fragments, short ssDNA fragments, short RNA fragments, short DNA/RNA hybrid fragments, oligos, and chimeric oligos.
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DNA Concentrator (Magnetic Beads)– BioDynami DNA Concentrator (Magnetic Beads) can be used to efficiently concentrate DNA/RNA from various samples with low concentration.
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cfDNA Purification Kit (Magnetic Beads) – BioDynami kit for cfDNA purification by removing genomic DNA contamination from cfDNA. The kit is effective for recovery of both cfDNA and genomic DNA separately.
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miRNA Purification Kit (tRNA Depletion)– BioDynami kit developed for microRNA (miRNA) purification by removal of tRNA, 5S RNA, and other larger RNA contaminations..
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Plasmid Purification Magnetic Beads (RNA Depletion)– BioDynami Plasmid Purification reagent removes RNA contamination and recovers plasmid DNA in the same step with a simple and fast beads-based protocol.
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RNA Contamination Removal Magnetic Beads (gDNA Purification)– BioDynami’s a simple reagent to completely remove RNA contamination in the genomic DNA samples with magnetic beads.
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DNA Normalization Magnetic Beads (NGS, PCR, gDNA)– BioDynami’s reagent to normalize NGS libraries, PCR fragments and genomic DNA samples with magnetic beads.
Nucleic Acid Extraction
Genomic DNA Extraction Kit (HMW, Magnetic Beads) for cells, blood, and tissues
The kit is a fast and simple reagent for isolating high molecular weight (HMW) genomic DNA from plants. The magnetic beads-based kit provides a consistent and reliable approach for high-quality plant DNA extraction due to the high-binding capacity and quick magnetic response time.
Genomic DNA Extraction Kit for Plants (HMW, Magnetic Beads)
The kit provides a reliable and fast process for extracting high molecular weight (HMW) genomic DNA from cells, blood, and tissues using Solid Phase Reversible Immobilization (SPRI) magnetic beads.
Disposable Pestle and Tube Set
The Disposable Pestle and Tube Set is ideal for homogenizing tissues and cells, and re-suspending precipitates such as protein pellets or DNA pellets in solutions.
Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)
The kit was developed for the extraction of plasmid DNA from small batch of bacterial cultures using magnetic beads. The kit can be used for high throughput and low throughput extraction of plasmid DNA.
Bacterial DNA Extraction Kit (Magnetic Beads)
The Bacterial DNA Extraction Kit (Magnetic Beads) was developed for the bacterial genomic DNA extraction from bacterial cultures directly using magnetic beads from a wide variety of gram-negative and gram-positive bacterial species, as well as yeast and other fungi.
Comparison of Enzymes for DNA fragmentation
Enzymes
DNA Fragmentation
The enzymes were developed for genomic DNA fragmentation in a fast and simple way and are compatible with EDTA-free DNA and DNA resuspended in TE buffer.
- DNA Fragmentation Enzyme Mix – Fast enzymatic DNA fragmentation in 30-45 min. Only 3 min of hands-on time. Ideal for NGS application.
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DNA Fragmentation & Blunting Enzyme Mix – Blunting enzymes were incorporated in addition of the above DNA fragmentation reagent. Ideal for NGS application, PCR cloning and blunt end ligation.
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DNA Fragmentation & A-tailing Enzyme Mix – A-tailing enzymes were incorporated in addition of the above DNA fragmentation reagent. Ideal for NGS application, PCR cloning and TA ligation
DNA Ligation
BioDynami DNA Ligation Master Mix reagents are available for ligation of difficult types of DNA fragments with high efficiency.
- Blunt/TA Ligation Master Mix – For ligation of difficult types of DNA fragments such as blunt ends and single overhang ends.
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Rapid Ligation Master Mix – For 5-minutes quick ligation of various types of DNA fragments such as sticky ends and blunt ends.
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Sticky End Ligation Master Mix – For quick ligation of DNA fragments with sticky ends.
NGS Enzyme
BioDynami NGS enzymes are stand-alone modules and also can be combined to work together. The enzymes are in a ready-to-use master mix format.
- End Repair/dA-tailing Master Mix – The Master Mix uses a one-step reaction combining DNA blunting, 5’ phosphorylation, and 3′ dA-tailing extension to convert randomly fragmented DNA for subsequent ligation with library adaptors.
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NGS Ligation Enzyme – For ligation of DNA fragments with NGS library adaptors.
Proteinase
BioDynami Proteinase K Proteinase K is a serine protease that exhibits broad protein cleavage activity.
- Proteinase K – The Proteinase K for protein removal, PCR purification, nucleic acid extraction such as genomic DNA, RNA, and plasmid.
DNA Ladder
BioDynami DNA ladders are available from 20 bp to 20 kb. All are ready-to-load.
- 20 bp DNA Ladder – From 20 bp to 800 bp, works on both agarose gel and acrylamide gel.
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100 bp DNA Ladder – From 100 bp to 1000 bp. The 500-bp band with higher concentration is easily distinguishable from the others.
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1 kb DNA Ladder – From 1 kb to 10 kb. The 4 kb band with higher concentration is easily distinguishable from the others.
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1 kb Plus DNA Ladder– From 1 kb to 20 kb. The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others.
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6X DNA Loading Buffer – For loading DNA markers and samples on agarose or polyacrylamide gels. It contains two dyes, xylene cyanol FF and orange G for visual tracking of DNA migration.